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> Products > PCR Cloning > Staby Cloning
StabyCloning(TM) kit
Speed, Precision, and Mobility in Cloning of PCR Products
The StabyCloning(TM) kit is designed for the rapid, precise and efficient DNA cloning of PCR products. The complete cloning procedure is performed in one hour (including plating), the background is basically nil (the bacteria containing vectors without insert are killed), the PCR product is oreinted, the plasmid is stabilized, and the export of the insert to another vector is easily selescted.
PRINCIPLE
The CYS21 bacterium, included in the StabyCloning kit, contains a natural bacterial poison gene (encoding the poison protein CcdB) into its chromosome. A truncated, inactive, antidote gene (ccdA*) is present in the plasmid vector (pSTC1). This vector is linearized at the end of the truncated antidote gene. The ends of the vector are blunt. [...]
